Explore the meaning of anabolism and catabolism in this comprehensive guide to the physiological factors and hormones that impact muscle growth and muscle loss.
This Guide Teaches You:
  • Exactly what the term metabolism really means.
  • What the major factors are that impact catabolism and anabolism.
  • About the impact of insulin on muscle protein synthesis.
  • The role of nourishment upon growth hormone, and insulin and IGF-1 production
  • How testosterone plays a significant role in the growth and maintenance of skeletal muscle tissue.
  • About the three major estrogens produced in the steroidogenesis pathway of humans: estradiol, estrone and estriol.
  • Why thyroid hormones are a major regulator of the human metabolism.
  • Why stress hormones are not something that should be avoided/inhibited at all costs.

Two of the most frequently thrown around words in bodybuilding subculture are “anabolic” and “catabolic”. However, I’m guessing that the majority of laypeople don’t actually know much about these terms beyond the fact that the former refers to building and the latter to breaking down.

Given that the main focus of many physique competitors and athletes alike is to improve their body composition, muscular hypertrophy and fat loss are often their primary concerns. Therefore, it seems prudent to cover the basics of what exactly anabolism and catabolism mean in these respects. Moreover, it’s important to understand what these two classes of reactions mean in the grand scheme of things for living organisms.

This guide is intended to cover the major factors of the human endocrine system and their role in protein anabolism and catabolism. Carbohydrate and fatty acid metabolism will be covered in a separate guide, along with the role of anaerobic & aerobic exercise and blood flow.

1. What is Metabolism?

Metabolism is one of those terms that pretty much everyone knows (and uses) but yet few actually understand, so this section will serve to give you an elementary grasp of what exactly metabolism is.

All living organisms are made up of the simplest living unit—the cell. Yes, this means even simple microbial organisms are “alive”, granted humans are composed of an enormous number (think 100s of trillions) of cells and are highly encephalized while many microbes exist as a single cell (and don’t have brains). But I digress…

Back to the concern at hand, within these cells, chemical reactions are constantly occurring while utilizing and giving off energy in the process. These reactions are divided into two classes that we alluded to in the introduction, those being anabolic and catabolic; the former uses energy to build cell components and molecules while the latter gives off energy as they break down complex substrates.

Therefore, when we speak of metabolism, we are referring to the summation of all of these physiological reactions within the cell that are necessary to sustain life. A multitude of variables such as hormone signaling, physical activity, nutrient availability, and energy status affect how these reactions occur and when they take place. For now, just know that metabolism is a highly intricate system of reactions in cells that sustain life, and there is an inherent energy input & output from these processes (thus the need for nutrients).

2. Improving Body Composition

The goal of almost any individual in the gym is to improve their body composition (i.e. reduce body-fat levels and/or increase muscle mass). The conundrum is that improving body composition is a give-and-take process. In bodybuilding and the fitness subculture, many people become obsessed with the idea of simultaneously losing fat and building muscle.

However, these events are theoretically mutually exclusive as one scenario requires an energetic deficit and the other requires an energetic surplus. Thus, when I come across a trainer or some magic program that “guarantees” it will help you build muscle and lose fat at the same time, I know to steer clear as that is a pretty arrogant claim unless that individual plans to overcome thermodynamic laws.

So one way to think of improving body composition is like a see-saw between building muscle and losing fat—if you want to increase one then the other will have to decrease.

This is why the traditional approach for many gym-goers looking to improve their body composition is to alternate between periods of building muscle mass and losing fat; colloquially, people usually refer to these timeframes as “bulking” and “cutting”, respectively. The other option is to be in a “maintaining” phase (neither building/losing muscle nor losing/gaining fat).

Related: How Protein Consumption & Quality Impact Body Composition

So let’s take a look at what role protein anabolism and catabolism play when it comes to this predicament of improving one’s body composition.

Backside of shirtless, muscular man doing pullups in a gym.

3. Protein and Building Skeletal Muscle Tissue

Skeletal muscle tissue serves as the largest reservoir of amino acids in the human body. Many bodybuilders and health enthusiasts love discussing the topic of protein intake, mainly because proteins provide the “building blocks”/amino acids necessary to synthesize muscle tissue.

However, many people misconstrue the message being sent when someone refers to protein synthesis. Proteins are essential macromolecules that play a myriad of roles in humans; they are not solely relegated to muscle tissue and in fact, are quite ubiquitous throughout many body systems:

  • Whole-body protein turnover - this is a measurement of the synthesis and breakdown of protein in all organs, skeletal and non-skeletal
  • Skeletal muscle protein turnover - this is a measurement of the synthesis and breakdown throughout skeletal muscle tissue

When it comes to improving body composition, it should intuitively make sense to you that we are trying to build skeletal muscle tissue specifically, since we aren’t after, say, hypertrophy of kidney tissue (well, at least not chronically).  This isn’t to say that whole-body protein anabolism is “a bad thing” (since it’s actually a vital part of human existence) but just that exorbitant whole-body protein anabolism over a period of time can actually lead to enlarged organs and subsequent health issues.

4. Important Terminology

Before moving on, hopefully, those of you who get a bit confused with all the technical jargon will benefit from this quick overview of some terms that will be used throughout this guide:

  • Muscle protein synthesis - refers to the synthesis of protein in skeletal muscle tissue only
  • Muscle protein degradation - refers to breakdown/degradation of skeletal muscle tissue only
  • Protein turnover - a measurement that detects that balance between protein synthesis and protein degradation
  • Muscle protein anabolism - refers to a state in skeletal muscle tissue where synthesis exceeds degradation, and thus lean tissue is being built.
  • Muscle protein catabolism - refers to a state in skeletal muscle tissue where degradation exceeds synthesis, and thus lean tissue is being broken down
  • Hypertrophy - the growth of tissue (generally in reference to muscle)
  • Atrophy - tissue shrinkage; opposite of hypertrophy

5. Factors That Affect Muscle Protein Anabolism and Catabolism

Okay, so now we have arrived at the meat and potatoes of this guide and will get into what factors play a large role in protein anabolism and catabolism, which ultimately have ramifications on body composition. As alluded to earlier, anabolic reactions serve to build cellular components and molecules while catabolic reactions do the opposite. Also, recall that anabolic reactions require energy input and catabolic reactions give off energy. So we will breakdown how protein anabolic and catabolic reactions play a role with regards to building skeletal muscle tissue—one of the most pertinent components to improving body composition.

Here is a preview of the topics to be covered:

  • Amino acid pooling, transport, and oxidation
  • Insulin
  • Insulin-like Growth Factor-1 (IGF-1) and IGF-Binding Protein-3 (IGFBP-3)
  • Growth hormone
  • Androgenic hormones
  • Estrogenic hormones
  • Thyroid hormones
  • “Stress hormones” - glucocorticoids, glucagon, and catecholamines

Blonde woman doing planks in a fitness studio.

5a. Amino Acid Pooling, Transport, and Oxidation

As noted earlier, skeletal muscle tissue serves as the largest reservoir of amino acids in the body and makes up the largest mass of protein. There are essentially two amino acid pools we are concerned with here–the circulating pool and intracellular pool.

When the body is in a state of starvation (and other catabolic instances), amino acids are released from muscle tissue into circulation and utilized by other body tissues. On the contrary, when protein anabolism is necessary, amino acids can be actively transported from circulation into the intracellular space of muscle cells and incorporated into proteins (thus synthesizing new protein).

So in addition to the availability of intracellular amino acids, protein synthesis/anabolism is also regulated in part by the transport of amino acids into and out of muscle cells.

In animals (mainly carnivores) amino acids provide a generous amount of energy from the oxidation of amino acids. The oxidation of amino acids to ammonia and their subsequent carbon skeletons occurs when there is excessive protein in the diet or during states of starvation, carbohydrate restriction, and/or diabetes mellitus.

Ammonia is excreted as urea via the kidneys in humans, while the carbon skeletons of amino acids enter the citric acid cycle for the production of energy. Some people claim that the stress placed on the kidneys from high protein intake is a case against traditional “bodybuilding diets”, but even upwards of 2 grams of protein per pound of lean bodyweight appear to be safe for those with healthy renal function (though that is a rather exorbitant intake for most natural trainees).

5b. Insulin

Insulin is a peptide hormone secreted in the pancreas of humans mainly in response to elevations in blood sugar levels (since it acts as an up-regulator of glucose transport proteins). With the onset of a dramatic increase in type-2 diabetes in the United States, insulin has unfortunately found itself being cast down as the enemy of human physiology as we know it.

However, I can assure you that if your goal is to build a lean, muscular physique, then you’ll be well served to let insulin work its anabolic mojo rather than trying to avoid it all costs like so many anti-carb advocates suggest.

Related: Carbs Aren't Making You Fat - The Truth About Insulin

Insulin is one of the most potent anabolic hormones in the human body and acts to induce protein anabolism in the entire body when amino acids are replenished. The key here is that a state of hyperinsulinemia (elevated insulin levels) without concomitant availability of amino acids doesn’t appear to increase whole-body protein synthesis (though it does reduce the rate of whole-body protein breakdown).1, 2, 3

Moreover, while insulin does reduce whole-body protein breakdown, it does not modulate the ubiquitin system that is responsible for the regulation of muscle protein breakdown. Therefore, insulin is not a specific reducer of muscle protein breakdown.4

Research suggests that insulin doesn’t directly alter the rate of transmembrane transport of (most) amino acids but it rather increases muscle protein synthesis by drawing on the active intracellular pool of amino acids.5 The exceptions to this are amino acids that use sodium-potassium pumps (mainly alanine, leucine, and lysine) as insulin causes skeletal muscle cells to be hyperpolarized by activation of those pumps.2

This suggests that a state of hyperinsulinemia paralleled with a state of hyperaminoacidemia (elevated plasma amino acid levels) should be quite conducive to facilitating muscle protein synthesis. This is in fact why patients in a state of critical cachexia are often set up with an infusion of amino acids and insulin.

Insulin Summary:

All the scientific mumbo jumbo can sometimes cause us to lose sight of the bigger picture. The take-home message is that insulin is indeed a highly anabolic hormone that is conducive to skeletal muscle protein synthesis but that an exogenous source of amino acids is necessary to create this effect.

As noted above, a state of hyperinsulinemia and hyperaminoacidemia will facilitate muscle protein synthesis, and what better way to induce such a state than by simply ingesting protein and carbohydrates.

However, be careful not to misconstrue the message here to mean “the more insulin the better” as this doesn’t appear to be the case in physiological ranges. Research seems to indicate that while some insulin does amplify the muscle protein synthesis response to feeding, there is a point of saturation in which extra insulin doesn’t confer a more intense response.6

Many people believe that a superfluous rush of fast-acting carbohydrates along with whey protein is ideal, especially after weight training to maximize the muscle protein synthetic response. The reality is you don’t have to “spike” your insulin; a slow, transient insulin response (as seen with low glycemic load carbohydrates) will provide much the same muscle protein synthesis benefits as a rapid, acute surge.

5c. Insulin-like Growth Factor-1 (IGF-1) & IGF-Binding Protein-3 (IGFBP-3)

As you may have likely derived from the nomenclature, IGF-1 is a peptide hormone quite similar in molecular structure to insulin and has implications on the growth of humans. IGF-1 is produced mainly in the liver upon binding of growth hormone (GH) and acts either locally on select tissues (paracrine) or systemically (endocrine); thus, IGF-1 is a mediator of the effects of GH. IGF-1 is a potent initiator of the AKT signaling pathway in cells, which has ramifications on cell growth and proliferation.

For practicality purposes, it is important to consider the actions of IGFBP-3 since nearly all IGF-1 is bound to one of 6 protein complexes and IGFBP-3 makes up about 80% of all this binding.

It is suggested that IGF-1 has effects similar to insulin (at high concentrations) on protein metabolism due to its ability to bind and activate the insulin receptor, albeit at a much less potent rate (about 1/10th the potency of insulin).7

Therefore, it’s not surprising that IGF-1 administration promotes protein anabolism in skeletal muscle and the whole body.8, 9 A unique characteristic of IGFBP-3 is that it appears to inhibit skeletal muscle atrophy (i.e. it’s anti-catabolic).10

IGF-1/IGFBP-3 Summary:

Given that IGF-1/IGFBP-3 is beneficial with respect to stimulating protein anabolism and preserving skeletal muscle tissue in times of muscle wasting/cachexia, the most prudent question most people are probably wondering about is, “How do we increase circulating levels of these moieties?”

Well, several factors influence the amount of IGF-1/IGFBP-3 (and GH) present in the blood at any given moment, including, but not limited to:  genetics, biorhythms, age, exercise, nutrient status, stress, disease state, and ethnicity.

However, many people may assume that an increase in insulin will confer a subsequent elevation in IGF-1, which is not the case (remember, insulin and IGF-1 are structurally and somewhat mechanistically similar, but they’re produced in different fashions). Intuitively, since GH is ultimately what leads to the production of IGF-1 (roughly 6-8 hours post GH release/administration), it makes the most sense to focus on elevating endogenous GH levels (which we will discuss in the growth hormone section of this guide).

Editor's Note on Deer Antler Velvet supplements that claim to increase IGF-1: In recent years some supplement companies have tried to make the claim that deer antler velvet extracts are conducive to skeletal muscle growth and healing in humans due to the potent amount of IGF-1 contained in said extracts. However, don’t fall prey to the hype behind these supplements as IGF-1 is a peptide hormone, therefore any oral form of it will be rapidly cleaved in the gastrointestinal (GI) tract before it gets into circulation. This is why people who are type-1 diabetics have to inject insulin (also a peptide hormone) and can’t just take an oral form of it since that too would be degraded by the GI tract.

Close up of man doing TRX push ups.

5d. Growth Hormone (GH)

GH is a peptide hormone produced in the pituitary gland that stimulates cellular growth and reproduction. When subjects are well-nourished, GH stimulates the production of insulin from the pancreas and IGF-1 once it reaches the liver which subsequently promotes the growth of lean body mass, adipose tissue, and storage of glucose. During fasting and other catabolic states, GH predominantly stimulates the release and oxidation of free fatty acids for use as energy, thus preserving lean body mass and glycogen stores.11

GH seems to be one of the most misunderstood hormones in the body with some so-called “fitness gurus” claiming it isn’t anabolic at all or even beneficial from a health standpoint (which is quite an arrogant thing to say given the body of scientific evidence against such claims). GH does indeed have a variety of anabolic actions in the human body, but they are mechanistically different from those of insulin.  GH may be viewed as the primary anabolic hormone during stress and fasting, whereas insulin is the major anabolic hormone in the preprandial timeframe.

Research has shown that GH strongly inhibits amino acid oxidation (recall from earlier that amino acids may be oxidized for energy). Thus, GH acts to spare crucial amino acids in the amino acid pools, resulting in greater availability of those amino acids for incorporation into proteins.11

That being said, it appears that GH is a promoter of whole-body protein synthesis in the short-term and any increases in muscle protein synthesis from GH administration are likely the result of downstream paracrine (local) IGF-1 release. Both GH and testosterone (which we will cover later in this guide) increase paracrine IGF-1 which could be of benefit for anabolic effects in skeletal muscle tissue.

A curious finding is that in studies that give subjects exogenous doses of IGF-1, the expression of paracrine IGF-1 is suppressed, and thus no increase in muscle protein synthesis is observed.7, 12, 13 Therefore, the use of IGF-1 as a performance-enhancing drug does not seem very pragmatic, at least for someone looking to acquire more skeletal muscle tissue.

It has been shown that GH not only promotes protein synthesis but also inhibits protein degradation, and it is likely that this effect is seen in skeletal muscle tissue due to paracrine expression IGF-1.14 The reason for attributing these anabolic effects to GH is quite simply because GH subsequently creates IGF-1.

A final point to consider with regards to the anabolic actions of GH is that it accelerates the transport of several essential amino acids across the cell membrane, specifically those mediated by System L—the major transport system responsible for sodium-independent transport of neutral amino acids (such as leucine, isoleucine, and valine).15

Growth Hormone Summary:

GH is a highly complex hormone that’s under much investigation as many of its in vivo actions are yet to be elucidated.

To recap, GH is a potent hormone that promotes whole-body protein synthesis and decreases whole-body protein breakdown, and it is likely that those effects could be induced in skeletal muscle tissue as well once the downstream production of paracrine IGF-1 kicks in (hopefully more research will be directed towards this concern in the coming years).

GH also strongly inhibits the oxidation and increases the transmembrane transport, of important amino acids such as the branched-chain amino acids leucine, isoleucine, and valine. Also of note is that GH is a major influencer on fat loss since it promotes the use of free fatty acids for energy.

As was noted in the above IGF-1 section, a variety of factors play into when and how much GH is secreted. Given that GH is secreted in a pulsatile fashion (with about 50% of the total daily secretion occurring during deep sleep) it is worthwhile to consider the following list of GH secretion stimulators and inhibitors:

Stimulators of GH secretion:16-22

  • Sex hormones (androgens and estrogen)
  • Peptide hormones such as ghrelin and growth hormone-releasing hormone (GHRH)
  • L-DOPA, the precursor to the neurotransmitter Dopamine
  • Nicotinic acid (Vitamin B3)
  • Nicotinic receptor agonists
  • Somatostatin inhibitors
  • Fasting
  • Deep sleep
  • Intense exercise

Inhibitors of GH secretion:18, 23, 24, 25, 26 

  • Somatostatin
  • Hyperglycemia (e.g. carbohydrates in the bloodstream)
  • IGF-1 and GH (due to negative feedback inhibition on the pituitary gland)
  • Xenobiotics
  • Glucocorticoids
  • Certain sex hormone metabolites, such as dihydrotestosterone (DHT)

5e. Androgens

Many readers are likely familiar with the term anabolic androgenic steroid (AAS) that is often used in the media and fitness subculture. That right there tells you that androgens are indeed anabolic hormones, and they influence the development and maintenance of male sex organs and secondary sex characteristics.

There are several androgens produced in the adrenal glands of humans, but the main one we will focus on here is testosterone (mainly produced in the testes of males and ovaries of females) given that it is the primary male sex hormone and most potent natural/endogenously-produced anabolic steroid.

There is a significant body of evidence that shows testosterone plays a pivotal role in the growth and maintenance of skeletal muscle tissue. In studies administering hypogonadal men a replacement dose of testosterone, fat-free mass, skeletal muscle strength, and muscle protein synthesis all increase rather dramatically.27, 28, 29 This effect has also been replicated in trained athletes and normal, healthy men upon administration of pharmacological doses of various androgens.30, 31

It appears that mechanistically testosterone, similarly to GH, exerts part of its anabolic effects by decreasing amino acid oxidation (specifically leucine) and increasing their uptake into whole-body and skeletal muscle proteins.32

Furthermore, there appears to be a synergistic (but independent) anabolic effect between testosterone and GH, enhancing their benefits on skeletal muscle protein synthesis.33

Androgens Summary:

There are many reasons testosterone (and other androgens) are so heavily researched, and it’s quite clear that these compounds have a myriad of anabolic actions in the human body. Testosterone is a strong inhibitor of amino acid oxidation and increases whole-body and skeletal muscle protein synthesis (and also appears to have anti-proteolytic effects).34

Like GH and IGF-1, many factors play into modulating endogenous production of testosterone; below is a truncated list of some of these variables.

Positive effectors: 35, 36, 37, 38, 39, 40

Negative effectors: 26, 38, 39, 41, 42, 43

  • Obesity/Over-fatness
  • Lack of sleep
  • Diabetes (specifically insulin-resistant/type II diabetes)
  • Sedentary lifestyle/inactivity
  • Very-low fat diets
  • Prolonged aerobic/cardiovascular exercise
  • Excessive alcohol intake
  • Xenobiotics

Man and woman doing medicine ball sit ups in gym setting.

5f. Estrogenic Hormones

Estrogens are quite plainly the principal female sex hormones and responsible for the growth and maturation of female reproductive tissues, but they are still present in males (albeit at much lower concentrations). There are three major estrogens produced in the steroidogenesis pathway of humans: estradiol, estrone, and estriol. Estradiol, on a molar basis, is about 10 times more potent than estrone and 80 times more potent than estriol with respect to its estrogenic effects.

In females, most estrogens are produced in the ovaries via aromatization of androstenedione, while in males it is produced in small amounts in the testes and more so from aromatization of testosterone in fat cells.

Unlike all the hormones we’ve discussed up to this point, estrogens appear to have both anabolic and catabolic properties with regards to protein metabolism (mainly through the mediation of other hormones in the body).

Studies have shown that estrogens increase systemic GH levels and paracrine IGF-1, both of those being favorable characteristics for protein anabolism and anti-catabolism. [44, 45, 46] Moreover, estrogens promote water retention which is conducive to cell volumization and thus anabolism.

However, when excess estrogen is present it can be indirectly catabolic by blockading androgen receptors and down-regulating the production of gonadotropin-releasing hormone in the hypothalamus ultimately lowering the production of testosterone in the body.

Estrogen Summary:

Like with many other things in the health and fitness world, there is a balance to be found when it comes to your estrogen levels. Estrogens have a medley of important effects in the human body and even a few anabolic/anti-catabolic effects on protein metabolism.

Just be cautious as excess estrogen levels (especially in males) will likely decrease testosterone production and availability, thereby impeding the positive effects of testosterone on protein metabolism.

Some general health tips to balance your estrogen production include:47-50

  • Eating a well-balanced diet that has ample vitamins/minerals and adequate dietary fiber.
  • Limiting the intake of soy and phytoestrogens from plant products
  • Limiting alcohol intake as alcohol impairs the liver’s ability to metabolize estrogens
  • Maintaining a regular workout/exercise regimen
  • Maintain a healthy body weight, avoiding obesity or being severely underweight

5g. Thyroid Hormones

Thyroid hormones are a major regulator of metabolic rate and affect nearly every cell in the human body. The thyroid gland produces thyroxine (T4) and triiodothyronine (T3), with T4 being the prohormone of T3. On a molar basis, T3 is about 20 times as potent as T4 and is thus considered the “true” thyroid hormone (most T3 in circulation comes from the deiodination of T4).

Research seems to suggest that thyroid hormones increase both whole-body protein synthesis and degradation, but more so the latter than the former, resulting in a net catabolic effect on whole-body protein metabolism.51, 52

In general, thyroid hormones in normal physiological ranges play a basic role in regulating protein metabolism, and it’s improper to extrapolate the data from hyperthyroidism (synthetically induced in the studies) protocols to mean that a euthyroid state is inherently catabolic. That being said, there doesn’t appear to be any benefits to skeletal muscle or whole-body protein anabolism by increasing thyroid production or using exogenous thyroid to achieve a hyperthyroid state, and if anything, it will likely have a net catabolic effect.

Thyroid Summary:

Being that the main goal of this guide is to discuss the ramifications of these hormones/factors on protein metabolism specifically, the above section doesn’t discuss the role that thyroid hormones play in fat and carbohydrate metabolism. Just know that the catabolic nature of thyroid hormones means they would be favorable for fat loss due to the up-regulation of metabolic rate (thus many people who have hyperthyroidism are usually underweight and/or have a hard time gaining weight).

However, if your goal is anabolism (especially of skeletal muscle tissue) it doesn’t seem wise to manipulate your thyroid levels. This is to say that the best way to go for proper protein metabolism would be to simply maintain a euthyroid state/be within the normal physiological range.

Man in the overhead position of a jerk in a CrossFit-style gym.

5h. Stress Hormones

The term “stress hormones” is often used in research literature to refer to glucocorticoids (mainly cortisol), glucagon, and catecholamines (specifically epinephrine/adrenaline). This is primarily due to the fact that their secretion is stimulated, quite plainly, in response to stress (note that stress isn’t always a “bad” thing, which would be more properly termed distress).

Glucocorticoids are a class of steroid hormones produced in the adrenal glands that regulate metabolism, development, immune function, and cognition/alertness. The primary glucocorticoid produced in humans is the stress hormone cortisol. Cortisol is an essential hormone necessary to sustain life, but like with many other hormones, too much (or too little) of it can wreak havoc on the body.

Cortisol is often implicated in the process of muscle atrophy/loss since it mainly acts as a catabolic hormone with regard to its metabolic functions. During periods of undernourishment/fasting, cortisol acts to maintain nominal glucose concentrations in the blood by initiating gluconeogenesis. Often times this comes at the cost of degrading proteins in order to utilize amino acids as a substrate for the gluconeogenic process.

Glucagon is a peptide hormone produced in the pancreas that functions basically in reverse to insulin (e.g. it stimulates the release of glucose from the liver into the bloodstream when blood sugar drops). Similar to cortisol, glucagon influences gluconeogenesis and also glycogenolysis.

The final hormone in this triad is epinephrine/adrenaline (sometimes referred to as the “fight-or-flight” hormone). This hormone is produced in the central nervous system and adrenal glands and acts on pretty much all tissues in the body by binding adrenergic receptors. As with cortisol and glucagon, epinephrine stimulates glycogenolysis in the liver (and muscle).

Protein synthesis rates in skeletal muscle tissue appear to decrease somewhat dramatically in response to stress hormone infusions.53, 54, 55 It appears that during prolonged exposure to stress hormones muscle protein synthesis is impaired, leading to atrophy of muscle tissue.56

Also of note is that epinephrine and cortisol may inhibit insulin secretion, and recall from earlier that insulin is an anabolic hormone. Some research also suggests that cortisol blunts the synthesis of paracrine IGF-1, which as aforementioned would be counterproductive to the goal of protein anabolism.57

Stress Hormones Summary:

The take-home message here is not that these stress hormones are not some “evil” hormones that should be avoided/inhibited at all costs because frankly, that’s just not what you want (in fact they’re essential to life in many ways).

The data does seem to show that infusions of these hormones promote protein breakdown in most tissues throughout the body and stimulates the oxidation of amino acids. They may also impair protein synthesis after chronic exposure and blunt the release of insulin and paracrine IGF-1. Ultimately the summation of these actions results in a net catabolic effect.

However, don’t misconstrue this message to mean that acute bursts of these hormones (as seen during times of acute stress) are counterproductive to muscle growth since that is missing the bigger picture; again, stress hormones are a necessary part of human physiology. Unless you have abnormally high cortisol, glucagon, and epinephrine concentrations in the blood for prolonged periods of time (e.g. Cushing’s Syndrome, chronic stress, etc.) there probably isn’t much reason to fret over lowering or inhibiting these hormones as is just not practical or healthy.

6. Bringing It All Together

While this guide is fairly rife with scientific jargon, I hope it serves readers with an understandable overview of some major factors that affect protein metabolism. This is a complex topic and protein metabolism is a growing field of research, but that’s what makes it worthwhile to investigate and discuss.

This guide is not intended to promote the use of any of the compounds/hormones discussed herein without the supervision/permission of a trained physician. The options laid out in this guide to modulate the levels of some of these hormones are all meant to act via endogenous production, not exogenous administration.

Lastly, remember that many physiological processes are not black-and-white functions or on/off switches. It is always imperative that we take into account circumstances and the context of the situation at hand. It is impractical, and ill-advised, to discount individual variables that come into play when offering someone advice with regards to their diet, nutrition, and training.

Therefore, this guide is meant to serve as a broad overview of the factors that mediate protein metabolism and give you, the reader, the necessary information to devise the optimal eating and lifestyle habits to achieve your physique and performance goals.

  1. Gelfand RA, Barrett EJ. Effect of physiologic hyperinsulinemia on skeletal muscle protein synthesis and breakdown in man. J Clin Invest. 1987 Jul;80(1):1-6. doi: 10.1172/JCI113033. PMID: 3298320; PMCID: PMC442193.
  2. Biolo G, Declan Fleming RY, Wolfe RR. Physiologic hyperinsulinemia stimulates protein synthesis and enhances transport of selected amino acids in human skeletal muscle. J Clin Invest. 1995 Feb;95(2):811-9. doi: 10.1172/JCI117731. PMID: 7860765; PMCID: PMC295560.
  3. McNulty PH, Louard RJ, Deckelbaum LI, Zaret BL, Young LH. Hyperinsulinemia inhibits myocardial protein degradation in patients with cardiovascular disease and insulin resistance. Circulation. 1995 Oct 15;92(8):2151-6. doi: 10.1161/01.cir.92.8.2151. PMID: 7554195.
  4. Kettelhut IC, Wing SS, Goldberg AL. Endocrine regulation of protein breakdown in skeletal muscle. Diabetes Metab Rev. 1988 Dec;4(8):751-72. doi: 10.1002/dmr.5610040805. PMID: 3148443.
  5. Sakurai Y, Aarsland A, Herndon DN, Chinkes DL, Pierre E, Nguyen TT, Patterson BW, Wolfe RR. Stimulation of muscle protein synthesis by long-term insulin infusion in severely burned patients. Ann Surg. 1995 Sep;222(3):283-94; 294-7. doi: 10.1097/00000658-199509000-00007. PMID: 7677459; PMCID: PMC1234807.
  6. Koopman R, Beelen M, Stellingwerff T, Pennings B, Saris WH, Kies AK, Kuipers H, van Loon LJ. Coingestion of carbohydrate with protein does not further augment postexercise muscle protein synthesis. Am J Physiol Endocrinol Metab. 2007 Sep;293(3):E833-42. doi: 10.1152/ajpendo.00135.2007. Epub 2007 Jul 3. PMID: 17609259.
  7. Mauras N, Beaufrere B. Recombinant human insulin-like growth factor-I enhances whole body protein anabolism and significantly diminishes the protein catabolic effects of prednisone in humans without a diabetogenic effect. J Clin Endocrinol Metab. 1995 Mar;80(3):869-74. doi: 10.1210/jcem.80.3.7533772. PMID: 7533772.
  8. Fryburg DA. Insulin-like growth factor I exerts growth hormone- and insulin-like actions on human muscle protein metabolism. Am J Physiol. 1994 Aug;267(2 Pt 1):E331-6. doi: 10.1152/ajpendo.1994.267.2.E331. PMID: 8074213.
  9. Debroy MA, Wolf SE, Zhang XJ, Chinkes DL, Ferrando AA, Wolfe RR, Herndon DN. Anabolic effects of insulin-like growth factor in combination with insulin-like growth factor binding protein-3 in severely burned adults. J Trauma. 1999 Nov;47(5):904-10; discussion 910-1. doi: 10.1097/00005373-199911000-00015. PMID: 10568720.
  10. Zdanowicz MM, Teichberg S. Effects of insulin-like growth factor-1/binding protein-3 complex on muscle atrophy in rats. Exp Biol Med (Maywood). 2003 Sep;228(8):891-7. doi: 10.1177/153537020322800804. PMID: 12968060.
  11. Møller N, Jørgensen JO. Effects of growth hormone on glucose, lipid, and protein metabolism in human subjects. Endocr Rev. 2009 Apr;30(2):152-77. doi: 10.1210/er.2008-0027. Epub 2009 Feb 24. PMID: 19240267.
  12. Mauras N, O'Brien KO, Welch S, Rini A, Helgeson K, Vieira NE, Yergey AL. Insulin-like growth factor I and growth hormone (GH) treatment in GH-deficient humans: differential effects on protein, glucose, lipid, and calcium metabolism. J Clin Endocrinol Metab. 2000 Apr;85(4):1686-94. doi: 10.1210/jcem.85.4.6541. PMID: 10770216.
  13. Lieberman SA, Butterfield GE, Harrison D, Hoffman AR. Anabolic effects of recombinant insulin-like growth factor-I in cachectic patients with the acquired immunodeficiency syndrome. J Clin Endocrinol Metab. 1994 Feb;78(2):404-10. doi: 10.1210/jcem.78.2.7508949. PMID: 7508949.
  14. Gibney J, Wallace JD, Spinks T, Schnorr L, Ranicar A, Cuneo RC, Lockhart S, Burnand KG, Salomon F, Sonksen PH, Russell-Jones D. The effects of 10 years of recombinant human growth hormone (GH) in adult GH-deficient patients. J Clin Endocrinol Metab. 1999 Aug;84(8):2596-602. doi: 10.1210/jcem.84.8.5916. PMID: 10443645.
  15. Inoue Y, Copeland EM, Souba WW. Growth hormone enhances amino acid uptake by the human small intestine. Ann Surg. 1994 Jun;219(6):715-22; discussion 722-4. doi: 10.1097/00000658-199406000-00016. PMID: 8203982; PMCID: PMC1243230.
  16. Wren AM, Small CJ, Ward HL, Murphy KG, Dakin CL, Taheri S, Kennedy AR, Roberts GH, Morgan DG, Ghatei MA, Bloom SR. The novel hypothalamic peptide ghrelin stimulates food intake and growth hormone secretion. Endocrinology. 2000 Nov;141(11):4325-8. doi: 10.1210/endo.141.11.7873. PMID: 11089570.
  17. Lin-Su K, Wajnrajch MP. Growth Hormone Releasing Hormone (GHRH) and the GHRH Receptor. Rev Endocr Metab Disord. 2002 Dec;3(4):313-23. doi: 10.1023/a:1020949507265. PMID: 12424433.
  18. Meinhardt UJ, Ho KK. Modulation of growth hormone action by sex steroids. Clin Endocrinol (Oxf). 2006 Oct;65(4):413-22. doi: 10.1111/j.1365-2265.2006.02676.x. PMID: 16984231.
  19. Alba-Roth J, Müller OA, Schopohl J, von Werder K. Arginine stimulates growth hormone secretion by suppressing endogenous somatostatin secretion. J Clin Endocrinol Metab. 1988 Dec;67(6):1186-9. doi: 10.1210/jcem-67-6-1186. PMID: 2903866.
  20. Van Cauter E, Latta F, Nedeltcheva A, Spiegel K, Leproult R, Vandenbril C, Weiss R, Mockel J, Legros JJ, Copinschi G. Reciprocal interactions between the GH axis and sleep. Growth Horm IGF Res. 2004 Jun;14 Suppl A:S10-7. doi: 10.1016/j.ghir.2004.03.006. PMID: 15135771.
  21. Nørrelund H. The metabolic role of growth hormone in humans with particular reference to fasting. Growth Horm IGF Res. 2005 Apr;15(2):95-122. doi: 10.1016/j.ghir.2005.02.005. PMID: 15809014.
  22. Quabbe HJ, Luyckx AS, L'age M, Schwarz C. Growth hormone, cortisol, and glucagon concentrations during plasma free fatty acid depression: different effects of nicotinic acid and an adenosine derivative (BM 11.189). J Clin Endocrinol Metab. 1983 Aug;57(2):410-4. doi: 10.1210/jcem-57-2-410. PMID: 6345570.
  23. Low LC. Growth hormone-releasing hormone: clinical studies and therapeutic aspects. Neuroendocrinology. 1991;53 Suppl 1:37-40. doi: 10.1159/000125793. PMID: 1901390.
  24. Guillemin R, Gerich JE. Somatostatin: physiological and clinical significance. Annu Rev Med. 1976;27:379-88. doi: 10.1146/annurev.me.27.020176.002115. PMID: 779605.
  25. Allen DB. Growth suppression by glucocorticoid therapy. Endocrinol Metab Clin North Am. 1996 Sep;25(3):699-717. doi: 10.1016/s0889-8529(05)70348-0. PMID: 8879994.
  26. Scarth JP. Modulation of the growth hormone-insulin-like growth factor (GH-IGF) axis by pharmaceutical, nutraceutical and environmental xenobiotics: an emerging role for xenobiotic-metabolizing enzymes and the transcription factors regulating their expression. A review. Xenobiotica. 2006 Feb-Mar;36(2-3):119-218. doi: 10.1080/00498250600621627. PMID: 16702112.
  27. Urban RJ, Bodenburg YH, Gilkison C, Foxworth J, Coggan AR, Wolfe RR, Ferrando A. Testosterone administration to elderly men increases skeletal muscle strength and protein synthesis. Am J Physiol. 1995 Nov;269(5 Pt 1):E820-6. doi: 10.1152/ajpendo.1995.269.5.E820. PMID: 7491931.
  28. Brodsky IG, Balagopal P, Nair KS. Effects of testosterone replacement on muscle mass and muscle protein synthesis in hypogonadal men--a clinical research center study. J Clin Endocrinol Metab. 1996 Oct;81(10):3469-75. doi: 10.1210/jcem.81.10.8855787. PMID: 8855787.
  29. Bhasin S, Storer TW, Berman N, Yarasheski KE, Clevenger B, Phillips J, Lee WP, Bunnell TJ, Casaburi R. Testosterone replacement increases fat-free mass and muscle size in hypogonadal men. J Clin Endocrinol Metab. 1997 Feb;82(2):407-13. doi: 10.1210/jcem.82.2.3733. PMID: 9024227.
  30. Young NR, Baker HW, Liu G, Seeman E. Body composition and muscle strength in healthy men receiving testosterone enanthate for contraception. J Clin Endocrinol Metab. 1993 Oct;77(4):1028-32. doi: 10.1210/jcem.77.4.8408450. PMID: 8408450.
  31. Alén M, Häkkinen K, Komi PV. Changes in neuromuscular performance and muscle fiber characteristics of elite power athletes self-administering androgenic and anabolic steroids. Acta Physiol Scand. 1984 Dec;122(4):535-44. doi: 10.1111/j.1748-1716.1984.tb07542.x. PMID: 6524396.
  32. Ferrando AA, Tipton KD, Doyle D, Phillips SM, Cortiella J, Wolfe RR. Testosterone injection stimulates net protein synthesis but not tissue amino acid transport. Am J Physiol. 1998 Nov;275(5):E864-71. doi: 10.1152/ajpendo.1998.275.5.E864. PMID: 9815007.
  33. Gibney, J., et al. "Testosterone enhances the effect of growth hormone (GH) to increase IGF-I but exerts an anabolic effect that is independent of GH action." (2003).
  34. Vingren JL, Kraemer WJ, Ratamess NA, Anderson JM, Volek JS, Maresh CM. Testosterone physiology in resistance exercise and training: the up-stream regulatory elements. Sports Med. 2010 Dec 1;40(12):1037-53. doi: 10.2165/11536910-000000000-00000. PMID: 21058750.
  35. Pilz S, Frisch S, Koertke H, Kuhn J, Dreier J, Obermayer-Pietsch B, Wehr E, Zittermann A. Effect of vitamin D supplementation on testosterone levels in men. Horm Metab Res. 2011 Mar;43(3):223-5. doi: 10.1055/s-0030-1269854. Epub 2010 Dec 10. PMID: 21154195.
  36. Jiang M, Xin J, Zou Q, Shen JW. A research on the relationship between ejaculation and serum testosterone level in men. J Zhejiang Univ Sci. 2003 Mar-Apr;4(2):236-40. doi: 10.1631/jzus.2003.0236. PMID: 12659241.
  37. Hulmi JJ, Ahtiainen JP, Selänne H, Volek JS, Häkkinen K, Kovanen V, Mero AA. Androgen receptors and testosterone in men--effects of protein ingestion, resistance exercise and fiber type. J Steroid Biochem Mol Biol. 2008 May;110(1-2):130-7. doi: 10.1016/j.jsbmb.2008.03.030. Epub 2008 Mar 30. PMID: 18455389.
  38. Zumoff B, Strain GW, Miller LK, Rosner W, Senie R, Seres DS, Rosenfeld RS. Plasma free and non-sex-hormone-binding-globulin-bound testosterone are decreased in obese men in proportion to their degree of obesity. J Clin Endocrinol Metab. 1990 Oct;71(4):929-31. doi: 10.1210/jcem-71-4-929. PMID: 2401718.
  39. Andersen ML, Alvarenga TF, Mazaro-Costa R, Hachul HC, Tufik S. The association of testosterone, sleep, and sexual function in men and women. Brain Res. 2011 Oct 6;1416:80-104. doi: 10.1016/j.brainres.2011.07.060. Epub 2011 Aug 6. PMID: 21890115.
  40. D'Aniello A, Di Cosmo A, Di Cristo C, Annunziato L, Petrucelli L, Fisher G. Involvement of D-aspartic acid in the synthesis of testosterone in rat testes. Life Sci. 1996;59(2):97-104. doi: 10.1016/0024-3205(96)00266-4. PMID: 8699926.
  41. Heinz A, Rommelspacher H, Gräf KJ, Kürten I, Otto M, Baumgartner A. Hypothalamic-pituitary-gonadal axis, prolactin, and cortisol in alcoholics during withdrawal and after three weeks of abstinence: comparison with healthy control subjects. Psychiatry Res. 1995 Jan 31;56(1):81-95. doi: 10.1016/0165-1781(94)02580-c. PMID: 7792345.
  42. Boyanov MA, Boneva Z, Christov VG. Testosterone supplementation in men with type 2 diabetes, visceral obesity and partial androgen deficiency. Aging Male. 2003 Mar;6(1):1-7. PMID: 12809074.
  43. Daly W, Seegers CA, Rubin DA, Dobridge JD, Hackney AC. Relationship between stress hormones and testosterone with prolonged endurance exercise. Eur J Appl Physiol. 2005 Jan;93(4):375-80. doi: 10.1007/s00421-004-1223-1. Epub 2004 Nov 20. PMID: 15618989.
  44. Kahlert S, Nuedling S, van Eickels M, Vetter H, Meyer R, Grohe C. Estrogen receptor alpha rapidly activates the IGF-1 receptor pathway. J Biol Chem. 2000 Jun 16;275(24):18447-53. doi: 10.1074/jbc.M910345199. PMID: 10749889.
  45. Cook DM, Ludlam WH, Cook MB. Route of estrogen administration helps to determine growth hormone (GH) replacement dose in GH-deficient adults. J Clin Endocrinol Metab. 1999 Nov;84(11):3956-60. doi: 10.1210/jcem.84.11.6113. PMID: 10566634.
  46. Friend KE, Hartman ML, Pezzoli SS, Clasey JL, Thorner MO. Both oral and transdermal estrogen increase growth hormone release in postmenopausal women--a clinical research center study. J Clin Endocrinol Metab. 1996 Jun;81(6):2250-6. doi: 10.1210/jcem.81.6.8964860. PMID: 8964860.
  47. Pischon T, Nöthlings U, Boeing H. Obesity and cancer. Proc Nutr Soc. 2008 May;67(2):128-45. doi: 10.1017/S0029665108006976. PMID: 18412987.
  48. Schiessl H, Frost HM, Jee WS. Estrogen and bone-muscle strength and mass relationships. Bone. 1998 Jan;22(1):1-6. doi: 10.1016/s8756-3282(97)00223-8. PMID: 9437507.
  49. Kendall B, Eston R. Exercise-induced muscle damage and the potential protective role of estrogen. Sports Med. 2002;32(2):103-23. doi: 10.2165/00007256-200232020-00003. PMID: 11817996.
  50. Adlercreutz CH, Goldin BR, Gorbach SL, Höckerstedt KA, Watanabe S, Hämäläinen EK, Markkanen MH, Mäkelä TH, Wähälä KT, Adlercreutz T. Soybean phytoestrogen intake and cancer risk. J Nutr. 1995 Mar;125(3 Suppl):757S-770S. doi: 10.1093/jn/125.3_Suppl.757S. Erratum in: J Nutr 1995 Jul;125(7):1960. PMID: 7884562.
  51. Tsalikian E, Lim VS. L-triiodothyronine at a slightly over physiologic dose increases leucine flux, which suggests an increase in protein degradation in normal subjects. J Lab Clin Med. 1989 Aug;114(2):171-5. PMID: 2754304.
  52. Tauveron I, Charrier S, Champredon C, Bonnet Y, Berry C, Bayle G, Prugnaud J, Obled C, Grizard J, Thiéblot P. Response of leucine metabolism to hyperinsulinemia under amino acid replacement in experimental hyperthyroidism. Am J Physiol. 1995 Sep;269(3 Pt 1):E499-507. doi: 10.1152/ajpendo.1995.269.3.E499. PMID: 7573427.
  53. McNurlan MA, Sandgren A, Hunter K, Essén P, Garlick PJ, Wernerman J. Protein synthesis rates of skeletal muscle, lymphocytes, and albumin with stress hormone infusion in healthy man. Metabolism. 1996 Nov;45(11):1388-94. doi: 10.1016/s0026-0495(96)90120-1. PMID: 8931644.
  54. Gore DC, Jahoor F, Wolfe RR, Herndon DN. Acute response of human muscle protein to catabolic hormones. Ann Surg. 1993 Nov;218(5):679-84. doi: 10.1097/00000658-199321850-00015. PMID: 8239784; PMCID: PMC1243041.
  55. Rooyackers OE, Nair KS. Hormonal regulation of human muscle protein metabolism. Annu Rev Nutr. 1997;17:457-85. doi: 10.1146/annurev.nutr.17.1.457. PMID: 9240936.
  56. Paddon-Jones D, Sheffield-Moore M, Cree MG, Hewlings SJ, Aarsland A, Wolfe RR, Ferrando AA. Atrophy and impaired muscle protein synthesis during prolonged inactivity and stress. J Clin Endocrinol Metab. 2006 Dec;91(12):4836-41. doi: 10.1210/jc.2006-0651. Epub 2006 Sep 19. PMID: 16984982.
  57. McCarthy TL, Centrella M, Canalis E. Cortisol inhibits the synthesis of insulin-like growth factor-I in skeletal cells. Endocrinology. 1990 Mar;126(3):1569-75. doi: 10.1210/endo-126-3-1569. PMID: 1689654.
Nicole B
Posted on: Wed, 03/08/2023 - 08:50

One of the best we’ll understand information I have read thus far ! Finally information about estrogen..and muscle .. thank you

Posted on: Thu, 07/22/2021 - 19:27

Thanks for sharing this blog post, it helps a lot.

Posted on: Tue, 04/03/2018 - 13:32

Great article! I thought it was broken down nicely and it helped on my paper for proteins (catabolism and anabolism). Thank you for sharing!

Posted on: Sun, 08/03/2014 - 01:21

Great article Elliot and thanks for taking the time to write and share. Special thanks to explain the science it simple and easy to understand format plus sharing summaries after section so to get complete picture. Can't wait for the next guide on carbs & fatty acids

Posted on: Mon, 08/04/2014 - 15:27

Thanks much for the feedback and taking the time to read Philip, I always appreciate it!

Posted on: Thu, 07/31/2014 - 15:02

THE best article of it's kind to date - Great work, Elliot!

Posted on: Thu, 07/31/2014 - 22:53

Thank you Dustin, I appreciate you taking the time to read and comment!

Posted on: Thu, 07/31/2014 - 12:43

Nice article

Posted on: Tue, 07/29/2014 - 18:59

Elliot. I'm a type 1 diabetic, and I've been a member of this site for about 4 months. I take insulin injections before meals, and 1 long acting insulin injection at night.

How does this affect my ability to grow muscle, as opposed to someone with a healthy pancreas?

Posted on: Tue, 07/29/2014 - 22:22

This is a great question Nathan, and one that I have actually pondered in the past. The research I've come across that examines the protein synthetic responses to endogenously produced insulin and exogenous administration of insulin actually suggest that the exogenous forms are more efficient.

There's a few "theories" on why exogenous insulin acts this way, but the one that makes most sense at this time (to me at least) is that exogenous insulin creates a monophasic release vs. endogenous insulin which is secreted in a biphasic fashion.

In fact, this study (cited below) found that burn patients administered insulin actually had more efficient protein synthesis responses than the control group and it appears to be due to increased reuse of intracellular amino acids.

1. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1191603/

Ultimately, I would surmise that despite being insulin-dependent, you shouldn't have any issues building muscle mass (at least not issues related to being a type-1 diabetic).

I hope this helps!

Caleb L Amundson
Posted on: Tue, 07/29/2014 - 15:51

Unbelievably informative article. Thanks Elliot

Posted on: Tue, 07/29/2014 - 22:00

Thanks for reading Caleb!